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CURRICULUM VITAE AMBEY SHREE SHUKLA DATE OF BIRTH: 7th December, 1978 NATIONALITY: Indian IMMIGRANT STATUS IN MEXICO: FM 3 (With Lucrative Activities) ADRESS FOR COMMUNICATION: AV. TAMAULIPUS # 1150 EDIF: C-02 DEPTO: 006 COL: CORPUS CRISTI DEL: ALVARO OBREGON MEXICO CITY MEXICO, C.P. 01401 ACADEMIC QUALIFICATIONS Degree(s) Universities/Colleges Dates Grade/Class Attended obtained [login to view URL]. Biology Department of Biology July1996- Jun.1999 First D.D.U. Gorakhpur University Gorakhpur-273016, India [login to view URL]. Zoology Department of Zoology July 2000- Jun.2002 First D.D.U. Gorakhpur University (10/10) * Gorakhpur-273016, India M.Phil Biotech. Department of Biophysics Aug. 2003-Oct.2004 First University of Delhi New Delhi-110021, India *As certified by DGIRE, Universidad Nacional Autónoma de México (UNAM), México City, México. TECHNICAL EXPERIENCE Ø Purification of different channel forming proteins like gap junctions and voltage dependent anion channels (VDAC). Ø Neuronal cell culture. Ø Bacterial Culture. Ø SDS-PAGE. Ø Lipid Bilayer Experiment Techniques (Black Lipid Membrane). Ø Voltage Clamp Experiments in Black Lipid Membrane. Ø Analysis of Electrophysiological data by using different software (Axoscope and pClamp 8.0) Ø Preparations of Liposome, Small Unilamellar Vesicles. Ø Western Blotting. Ø Paper Chromatography and Ion Exchange Chromatography. Ø Preparation of permanent slides (microtomy) and its detailed study for- Protein, Glycogen, DNA and RNA in different animal tissues. Ø Colorimetric determination of Glycogen, Protein, Fat, DNA and RNA in different animal tissues by using Absorption Spectrophotometer. Ø Colorimetric analysis of residual water Ø Determination of respiration rate of different animals. Ø Study of functional properties of Cardiac muscles, pharmacologically (using Acetylcholine and Adrenaline). Ø Study of different stages of different types of cell division. Ø Purification of water by Ultrasonication techniques. Ø COD (Chemical Oxygen Demand) determination of water. Ø Estimation of Phenol, Oils and Fats in waste water. Job Experience Institution Department Job Status Duration Westhill Institute Middle/High school Teacher (in English) Current México City, México 9-12th grade (Biological Sci./Chemistry/ Earth/Environmental Sci.) Tec. De Monterrey Department of Professor Current CampusSanta Fe, D.F Mecatronics Sci. Technology and Sustainable development Instituto Ovalle Monday Escuela Secundaria Teacher (in English) 12 months México City, México 3º grade (Environmental Edu.) Tecnológico de Monterrey Environmental Quality Professional Assistant 10 months Estado de México, México Research Centre Instituto Nacional Neurophysiology Laboratory Technician 4 months de Psiquiatría México D.F., México Computer Skills • Diploma in Computer Programming and Management. • Languages known: - FOX, COBOL and C++. • Working Knowledge in MS-Office, MS-PowerPoint, Adobe Photoshop, Micro cal Origin for Graphics. M.Phil. Dissertation The dissertation work carried out during my M.Phil.- Biotechnology at the Department Of Biophysics, University of Delhi-South Campus, New Delhi, India was entitled- “Asymmetric Voltage-dependence of Voltage Dependent Anion Channel: An Electrophysiological Approach.” Publication: Kartikeya Shukla, Ambey Shree and A. K. Tripathi. ´´Implication and challenges from pollutants to the Biodiversity.`` Souvenir 2002, Academy of Environmental Biology, 83 (PP), p.20. PERSONAL PROFILE Marital status Married Languages known English, Hindi and Spanish PLACE: Mexico City AMBEY SHREE SHUKLA