Ct to Viral Expression Conversion

$30-250 USD

Completed

Posted 6 days ago

$30-250 USD

Paid on delivery

I have an Excel spreadsheet that contains raw Cycle threshold (Ct) values for both the Citrus yellow vein clearing virus target sequence and my chosen housekeeping gene, Nad5. I need those Ct data converted into meaningful viral expression values so I can compare virus titres across my plant samples. What I’m after is a clean, well-documented workflow that takes the raw Ct numbers, normalises them against Nad5, and outputs relative expression (e.g., 2^-ΔΔCt or similar) for each sample. Please include an ANOVA so I can see whether titre differences among my treatment groups are statistically significant; if you believe another test would add value, feel free to flag it, but ANOVA results are required. Deliverables • A processed spreadsheet (or CSV) with ΔCt, ΔΔCt and final relative expression columns • A short methods write-up explaining formulas, software, and any assumptions • ANOVA summary table and a simple plot visualising group differences • Optional: a small script (R, Python or Excel macro) so I can rerun the analysis on future data Graphs or tables for scientific publication. Accuracy and transparency are crucial—I will double-check calculations, so please leave the formulas visible or fully commented. If anything in my raw file needs cleaning or clarification, let me know early so we keep the turnaround quick.

Project ID: 40689617

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Active 6 days ago

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@hudex

hello sir, I am a professional Excel data analysis data processing and data scientist expert and can do your task perfectly because I have done lots of similar tasks in my 7 years of experience and also I am in top 1% here so just text me so I can help you out with your task kind regards

$100 USD in 1 day

4.7

(48 reviews)

6.6

19 freelancers are bidding on average $100 USD for this job

@JustinJcob

I got you! I can convert your raw Ct spreadsheet for Citrus yellow vein clearing virus and Nad5 into a transparent ΔCt/ΔΔCt workflow with relative expression, ANOVA results, and publication-ready tables/plots. I’m ready to handle the full analysis carefully: data cleaning checks, formula-visible spreadsheet/CSV, documented assumptions, and an optional R/Python script for reruns. I’m young, a fast learner, available 24/7, and I’ll keep everything accurate and easy to verify. Get the demo first before you pay. A couple of quick questions: which sample/treatment group should be used as the calibrator/control for ΔΔCt, and do you have technical replicates that should be averaged before ANOVA or treated separately? Let’s chat so we can confirm these details and I can start immediately. Kind regards, Haroon Z

$250 USD in 1 day

5.0

(11 reviews)

5.3

@Georgeoustar

Hi, there! I understand you need the raw Ct data converted into accurate, fully traceable viral expression results, with Nad5 normalization, ΔCt, ΔΔCt, relative expression, statistical testing, and publication ready outputs. I’ve handled similar qPCR analysis involving housekeeping gene normalization, technical and biological replicates, 2^-ΔΔCt calculations, ANOVA, post hoc comparisons, and scientific graphs. I can work directly from your Excel file, keep formulas visible, document every assumption, and provide a reusable analysis workflow. I’d be glad to review the workbook and confirm the statistical structure before processing. Best regards, George

$140 USD in 7 days

5.0

(2 reviews)

4.0

@huzaifa306

I can process your raw Ct data, normalize it against Nad5, calculate the relative expression and perform the required ANOVA with clear tables and graphs. I will provide a clean documented spreadsheet with formulas and a short methods explanation. I can also provide a simple Python or R script so you can repeat the analysis with future data.

$75 USD in 2 days